On the occasion of 25th anniversary of the program LIFE and 20th anniversary of the Regional Association For Nature Conservation And Sustainable Development an Open Day of the project Restoration and management of Danube floodplain habitats – LIFE14 NAT/SK/001306 took place on 17th of May 2017 from 8:30 till 21:00 in Waterworks museum in Bratislava. The venue attended members from various organisations from different sectors: state institutions (Ministry of Environment SR, State Nature Conservancy, Water Research Institute, National Forest Centre, Military Forests and Estates of the SR – Záhorie, Slovak Hydrometeorological Institute and others), members from European Commission in Slovakia, public institutions (mayors and members of municipalities Devín and Karlova Ves, Bratislava Water Company, Bratislava Self-Governing Region and others, private foundations (e.g. Danube Fund), non-profit organisations and civil associations (SOVS Bird Life Slovensko, Živica, Daphne, OZ Nase Karpaty and others), journalists, private companies, members of foreign partners (National park Donau Auen in Austria, National park Duna-Ipoly) and public. The main part of the program was presentation of realised activities in the Danube region (which is the project region), common achivements as well as lessons we learned from failures. In public discussion new challenges were drafted for following years in the field of nature conservation and biodiversity also in the context of the LIFE project. The main challenge and necessity for a constructive cooperation of all sector members was identified as the basics for the progress in nature conservation. It was agreed that also those who are not directly responsible for nature conservation according to the law should cooperate with those who are. The venue had positive feedback from the visitors.